Journal: Neuropsychopharmacology Reports

Article Title: Preliminary analysis of hippocampus synaptic apoptosis and microglial phagocytosis induced by severe restraint stress

doi: 10.1002/npr2.12298

Figure Lengend Snippet: We acquired images from CA3 stratum lucidum region in control and stressed male C‐57 BL6 mice using confocal laser scanning microscopy, subsequently for analysis converted and binarize them using image converter/analyzer. Scale bar, 5 μm. (A) representative converted (left side) and binarized (right side) images in control and stressed mice. (B) ratios of the area where VGLUT1 co‐localizes with caspase 3 to the VGLUT1+ area.10‐d water immersion restraint stress significantly increase the value (control: n = 4 animals, stress: n = 3 animals, t5 = 3.0265, P = .0292). Error bars, SD

Article Snippet: For evaluating local synaptic apoptosis, sections were double stained with rabbit anti‐cleaved caspse‐3 (1:300; Cell Signaling, #9664) and guinea pig anti‐ vesicular glutamate transporter1 (VGLUT1) antibody (1:2000; synaptic systems, #135304) for 6 days at 4°C.

Techniques: Control, Confocal Laser Scanning Microscopy

Journal: Neuropsychopharmacology Reports

Article Title: Preliminary analysis of hippocampus synaptic apoptosis and microglial phagocytosis induced by severe restraint stress

doi: 10.1002/npr2.12298

Figure Lengend Snippet: (A) Representative 3D reconstruction images obtained from CA3 stratum lucidum region in control and stressed male C‐57 BL6 mice. Iba1+ microglia and LAMP1+ structures are visualized at left side. Iba1+ microglia, LAMP1+ structures and VGLUT1+ structures are visualized at right side. Scale bar, 5 μm. (B) Ratios of the volume of the LAMP1+ structures contained in the Iba1+ microglia to the volume of the Iba1+ microglia. There was no significant difference between control and stressed groups in the ratio (control: n = 4 animals, stress: n = 3 animals, t5 = 0.8946, P = .412). Error bars, SD . (C) Ratios of the volume of engulfed VGLUT1 within LAMP1+ structures contained in the Iba1+ microglia to the volume of the Iba1+ microglia. There was no significant difference between control and stressed groups in the ratio (control: n = 4 animals, stress: n = 3 animals, t5 = 0.4414, P = .6773). Error bars, SD

Article Snippet: For evaluating local synaptic apoptosis, sections were double stained with rabbit anti‐cleaved caspse‐3 (1:300; Cell Signaling, #9664) and guinea pig anti‐ vesicular glutamate transporter1 (VGLUT1) antibody (1:2000; synaptic systems, #135304) for 6 days at 4°C.

Techniques: Control

Journal: Cell Reports

Article Title: Microglial control of neuronal development via somatic purinergic junctions

doi: 10.1016/j.celrep.2022.111369

Figure Lengend Snippet:

Article Snippet: Guinea pig anti-vGluT1 , Synaptic Systems , Cat# 135304; RRID: AB_887878.

Techniques: Recombinant, Plasmid Preparation, Electron Microscopy, TUNEL Assay, Software